LASER SCANNING CONFOCAL MICROSCOPE

The Zeiss LSM 510 META Confocal Laser Scanning Microscope system has a wealth of general functions for confocal image acquisition and analysis. In addition, the system is equipped with a heated stage for live analysis.
Some of the features of this system include the following:
•Colocalization of fluorescently-tagged components.
•Fluorescence Intensity Measurements of any given region of interest or profile.
•Z-Sectioning of biospecimens for Orthogonal analysis and generation of Projections.
•Time Lapse image acquisition of live samples.
•Fluorescence Resonance Energy Transfer (FRET) analysis of protein-protein interaction.
•Emission Fingerprinting for separation of overlapping emission spectra.


FEES
Zeiss LSM 510 META Confocal Laser Scanning Microscope
TRAINING/ORIENTATION $60/hr
CONFOCAL MICROSCOPE AND COMPUTER (Independent Use) $40/hr
COMPUTER ONLY (LSM Image Examiner)     $20/hr
A second copy of LSM Image Examiner is on the VOLOCITY computer in adjacent room.

All users must go thru a training/orientation session prior to using any of the instrumentation.
Minimum 1 hr. Estimated 2hr for new users.

Please contact Wandy Beatty for information or scheduling orientation/training sessions.
beatty@borcim.wustl.edu
362-4987

LASERS
Laser             Excitation Wavelength
Argon            458, 477, 488, 514 nm
HeNe1           543 nm
HeNe2           633 nm

MICROSCOPE
Zeiss Axiovert Microscope (inverted)

Objectives
OBJECTIVE
N.A
W.D.
(mm)
Coverslip
thickness (mm)
Plan-Apochromat
63X Oil
DIC
1.4
0.18
0.17
C-Apochromat
63X W Korr
DIC
1.2
0.24
0.14… 0.18
C-Apochromat
40X W Korr
DIC
1.2
0.29
0.14… 0.18
LD Achroplan
20X Korr
DIC
0.4
0.17
Plan-Neofluor
10X
0.3
5.6
0.17

Filter Sets
FITC, TxRed, and Cy5 filter sets for fluorescence screening.
Heated stage
For live imaging
Plates that have been used successfully on this system for live imaging.
MatTek Corporation
35mm plates with glass coverslip bottom
P35G-0-14-C (0.85-0.13 mm thickness, 14mm diameter glass)
P35G-0-10-C (0.85-0.13 mm thickness, 10mm diameter glass)
www.GlassBottomDishes.com

Bioptechs, Inc.
35mm dishes with glass coverslip bottom
04200415C Delta T Dish 0.17mm clear
www.bioptechs.com

FINAL IMAGES
All images will be in lsm format. These can be viewed with the LSM Image Examiner software available on the LSM Confocal computer and the Axiovskop/Axiovision computer. You may also view these on any PC using the LSM Image Browser which can be downloaded free from the Zeiss website.
Most users export their images as tif or jpg files, or as avi or mov for movie formats.