WIDE
FIELD FLUORESCENCE MICROSCOPE The Zeiss Axioskop 2 MOT Plus interacts with Axiovision for image capture and processing. Axiovision has a wealth of general functions for image optimization and documentation with the following additional modules: •Multichannel Acquisition software controls the motorized reflector turret allowing for rapid automatic acquisition of multiple channels with optimal exposure times for each channel. •Interactive Measurement for accurate measurement of distances, areas, and pixel intensity. •Z-Stack module controls the Z-drive of the motorized stage for Z-stack generation and orthogonal analysis. •3D Deconvolution for computational reduction of out-of-focus fluorescence in three-dimensional microscope images resulting in images with less noise, better definition, and higher resolution. FEES Zeiss Axioskop Fluorescence Microscope
All users must go thru a training/orientation session prior to using any of the instrumentation. Estimated 1/2hr. Please contact Wandy Beatty for information or scheduling orientation/training sessions. beatty@borcim.wustl.edu 362-4987 MICROSCOPE Zeiss Axioskop MOT Plus Microscope (upright) The refelector turret is automated allowing for automatic acquisition of multichannel images with Axiovision software. The stage is automated for precise Z-step acquisition for Z-stack analysis and 3D Deconvolution with Axiovision software. Objectives
Filter Sets DAPI, FITC, TxRed, and Cy5. CAMERA The system is equipped with an Axiocam MRm Monochrome Camera with the following features: •High 12-bit dynamics •18MHz digitalization •Maximum resolution in 1300 X 1030 pixels •Expanded spectral range •High sensitivity for near infrared FINAL IMAGES All images will be archived in zvi format which can be viewed in Axiovision. You may also view these on any PC using the Zeiss Axiovision Image Viewer which can be downloaded free from the Zeiss website. Most users export their images as tif or jpg files or as avi or mov for movie formats. |